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Monomeric site-specific nucleases for genome editing

Targeted manipulation of complex genomes often requires the introduction of a double-strand break at defined locations by site-specific DNA endonucleases. Here, we describe a monomeric nuclease domain derived from GIY-YIG homing endonucleases for genome-editing applications. Fusion of the GIY-YIG nu...

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Autors principals: Kleinstiver, Benjamin P., Wolfs, Jason M., Kolaczyk, Tomasz, Roberts, Alanna K., Hu, Sherry X., Edgell, David R.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3361397/
https://ncbi.nlm.nih.gov/pubmed/22566637
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1117984109
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