Biasing genome-editing events toward precise length deletions with an RNA-guided TevCas9 dual nuclease

The CRISPR/Cas9 nuclease is commonly used to make gene knockouts. The blunt DNA ends generated by cleavage can be efficiently ligated by the classical nonhomologous end-joining repair pathway (c-NHEJ), regenerating the target site. This repair creates a cycle of cleavage, ligation, and target site r...

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Библиографические подробности
Опубликовано в: :Proc Natl Acad Sci U S A
Главные авторы: Wolfs, Jason M., Hamilton, Thomas A., Lant, Jeremy T., Laforet, Marcon, Zhang, Jenny, Salemi, Louisa M., Gloor, Gregory B., Schild-Poulter, Caroline, Edgell, David R.
Формат: Artigo
Язык:Inglês
Опубликовано: National Academy of Sciences 2016
Предметы:
Biological Sciences
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5206545/
https://ncbi.nlm.nih.gov/pubmed/27956611
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1616343114
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