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Trapping and structure determination of an intermediate in the allosteric transition of aspartate transcarbamoylase

X-ray crystallography and small-angle X-ray scattering (SAXS) in solution have been used to show that a mutant aspartate transcarbamoylase exists in an intermediate quaternary structure between the canonical T and R structures. Additionally, the SAXS data indicate a pH-dependent structural alteratio...

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Bibliographic Details
Main Authors: Guo, Wenyue, West, Jay M., Dutton, Andrew S., Tsuruta, Hiro, Kantrowitz, Evan R.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2012
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3356622/
https://ncbi.nlm.nih.gov/pubmed/22547808
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1119683109
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