Random mutagenesis of the human immunodeficiency virus type-1 trans-activator of transcription (HIV-1 Tat).

A new method is described for the direct construction of randomly mutagenized genes by applying the polymerase chain reaction (PCR) to an oligonucleotide synthesized using doped nucleotide reservoirs. We have demonstrated the utility of this method by generating a library of mutant HIV-1 tat genes....

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Autores principales: Siderovski, D P, Matsuyama, T, Frigerio, E, Chui, S, Min, X, Erfle, H, Sumner-Smith, M, Barnett, R W, Mak, T W
Formato: Artigo
Lenguaje:Inglês
Publicado: 1992
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC334336/
https://ncbi.nlm.nih.gov/pubmed/1437550
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