Random mutagenesis of the human immunodeficiency virus type-1 trans-activator of transcription (HIV-1 Tat).

A new method is described for the direct construction of randomly mutagenized genes by applying the polymerase chain reaction (PCR) to an oligonucleotide synthesized using doped nucleotide reservoirs. We have demonstrated the utility of this method by generating a library of mutant HIV-1 tat genes....

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Siderovski, D P, Matsuyama, T, Frigerio, E, Chui, S, Min, X, Erfle, H, Sumner-Smith, M, Barnett, R W, Mak, T W
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1992
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334336/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1437550/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.20.5311
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