Caricamento...

Random mutagenesis of the human immunodeficiency virus type-1 trans-activator of transcription (HIV-1 Tat).

A new method is described for the direct construction of randomly mutagenized genes by applying the polymerase chain reaction (PCR) to an oligonucleotide synthesized using doped nucleotide reservoirs. We have demonstrated the utility of this method by generating a library of mutant HIV-1 tat genes....

Descrizione completa

Salvato in:
Dettagli Bibliografici
Autori principali: Siderovski, D P, Matsuyama, T, Frigerio, E, Chui, S, Min, X, Erfle, H, Sumner-Smith, M, Barnett, R W, Mak, T W
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1992
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC334336/
https://ncbi.nlm.nih.gov/pubmed/1437550
Tags: Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne! !