Random mutagenesis of the human immunodeficiency virus type-1 trans-activator of transcription (HIV-1 Tat).

A new method is described for the direct construction of randomly mutagenized genes by applying the polymerase chain reaction (PCR) to an oligonucleotide synthesized using doped nucleotide reservoirs. We have demonstrated the utility of this method by generating a library of mutant HIV-1 tat genes....

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Detaylı Bibliyografya
Yayımlandı:	Nucleic Acids Res
Asıl Yazarlar:	Siderovski, D P, Matsuyama, T, Frigerio, E, Chui, S, Min, X, Erfle, H, Sumner-Smith, M, Barnett, R W, Mak, T W
Materyal Türü:	Artigo
Dil:	Inglês
Baskı/Yayın Bilgisi:	Oxford University Press 1992
Online Erişim:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334336/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1437550/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.20.5311
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Random mutagenesis of the human immunodeficiency virus type-1 trans-activator of transcription (HIV-1 Tat).

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