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Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity.
Two thermostable DNA polymerases with proofreading activity--Vent DNA polymerase and Pfu DNA polymerase--have attracted recent attention, mainly because of their enhanced fidelities during amplification of DNA sequences by the polymerase chain reaction. A severe disadvantage for their practical appl...
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| Veröffentlicht in: | Nucleic Acids Res |
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| 1. Verfasser: | |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1992
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| Online Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334000/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1641322/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.14.3551 |
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