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Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity.
Two thermostable DNA polymerases with proofreading activity--Vent DNA polymerase and Pfu DNA polymerase--have attracted recent attention, mainly because of their enhanced fidelities during amplification of DNA sequences by the polymerase chain reaction. A severe disadvantage for their practical appl...
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| Autor principal: | |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1992
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC334000/ https://ncbi.nlm.nih.gov/pubmed/1641322 |
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