Predicting the success of primer extension genotyping assays using statistical modeling

Using an empirical panel of more than 20 000 single base primer extension (SNP-IT) assays we have developed a set of statistical scores for evaluating and rank ordering various parameters of the SNP-IT reaction to facilitate high-throughput assay primer design with improved likelihood of success. Ea...

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Main Authors: Yuryev, Anton, Huang, JianPing, Pohl, Mark, Patch, Robert, Watson, Felicia, Bell, Peter, Donaldson, Miriam, Phillips, Michael S., Boyce-Jacino, Michael T.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2002
Teme:
NAR Methods Online
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC137986/
https://ncbi.nlm.nih.gov/pubmed/12466563
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