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Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity.

Two thermostable DNA polymerases with proofreading activity--Vent DNA polymerase and Pfu DNA polymerase--have attracted recent attention, mainly because of their enhanced fidelities during amplification of DNA sequences by the polymerase chain reaction. A severe disadvantage for their practical appl...

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Detalles Bibliográficos
Autor Principal: Skerra, A
Formato: Artigo
Idioma:Inglês
Publicado: 1992
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC334000/
https://ncbi.nlm.nih.gov/pubmed/1641322
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