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Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity.

Two thermostable DNA polymerases with proofreading activity--Vent DNA polymerase and Pfu DNA polymerase--have attracted recent attention, mainly because of their enhanced fidelities during amplification of DNA sequences by the polymerase chain reaction. A severe disadvantage for their practical appl...

詳細記述

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書誌詳細
出版年:Nucleic Acids Res
第一著者: Skerra, A
フォーマット: Artigo
言語:Inglês
出版事項: Oxford University Press 1992
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334000/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1641322/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.14.3551
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