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NON-REDUCING ALKALINE SOLUBILIZATION AND RAPID ON-COLUMN REFOLDING OF RECOMBINANT PRION PROTEIN

Mature prion protein (PrP) is a 208-residue polypeptide that contains a single disulfide bond. We report an alternative method to purify recombinant mouse PrP produced in Escherichia coli. Bacterial inclusion bodies were solubilized in a buffer containing 2 M urea at pH 12.5. The solubilized protein...

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Main Authors: Walsh, Daniel J., Noble, Geoffrey P., Piro, Justin R., Supattapone, Surachai
Formato: Artigo
Idioma:Inglês
Publicado: 2012
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3329176/
https://ncbi.nlm.nih.gov/pubmed/22239709
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/10826068.2011.564256
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