Minimizing the Impact of Photoswitching of Fluorescent Proteins on FRAP Analysis

Fluorescence recovery after photobleaching (FRAP) is a widely used imaging technique for measuring the mobility of fluorescently tagged proteins in living cells. Although FRAP presumes that high-intensity illumination causes only irreversible photobleaching, reversible photoswitching of many fluores...

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Hlavní autoři: Mueller, Florian, Morisaki, Tatsuya, Mazza, Davide, McNally, James G.
Médium: Artigo
Jazyk:Inglês
Vydáno: The Biophysical Society 2012
Témata:
Spectroscopy, Imaging, and Other Techniques
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3318116/
https://ncbi.nlm.nih.gov/pubmed/22500766
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2012.02.029
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