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Minimizing the Impact of Photoswitching of Fluorescent Proteins on FRAP Analysis
Fluorescence recovery after photobleaching (FRAP) is a widely used imaging technique for measuring the mobility of fluorescently tagged proteins in living cells. Although FRAP presumes that high-intensity illumination causes only irreversible photobleaching, reversible photoswitching of many fluores...
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| Main Authors: | , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
The Biophysical Society
2012
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3318116/ https://ncbi.nlm.nih.gov/pubmed/22500766 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2012.02.029 |
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