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Photoswitching-Free FRAP Analysis with a Genetically Encoded Fluorescent Tag
Fluorescence recovery after photobleaching (FRAP) is a widely used imaging technique for measuring protein dynamics in live cells that has provided many important biological insights. Although FRAP presumes that the conversion of a fluorophore from a bright to a dark state is irreversible, GFP as we...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2014
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4169462/ https://ncbi.nlm.nih.gov/pubmed/25233348 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0107730 |
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