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Photoswitching-Free FRAP Analysis with a Genetically Encoded Fluorescent Tag

Fluorescence recovery after photobleaching (FRAP) is a widely used imaging technique for measuring protein dynamics in live cells that has provided many important biological insights. Although FRAP presumes that the conversion of a fluorophore from a bright to a dark state is irreversible, GFP as we...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Morisaki, Tatsuya, McNally, James G.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Public Library of Science 2014
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4169462/
https://ncbi.nlm.nih.gov/pubmed/25233348
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0107730
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