Cross-Validating FRAP and FCS to Quantify the Impact of Photobleaching on In Vivo Binding Estimates

Binding can now be quantified in live cells, but the accuracy of such measurements remains uncertain. To address this uncertainty, we compare fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS) measurements of the binding kinetics of a transcription fact...

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Detalhes bibliográficos
Main Authors: Stasevich, Timothy J., Mueller, Florian, Michelman-Ribeiro, Ariel, Rosales, Tilman, Knutson, Jay R., McNally, James G.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2010
Assuntos:
Spectroscopy, Imaging, and Other Techniques
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2966010/
https://ncbi.nlm.nih.gov/pubmed/21044608
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.08.059
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