Cross-Validating FRAP and FCS to Quantify the Impact of Photobleaching on In Vivo Binding Estimates

Binding can now be quantified in live cells, but the accuracy of such measurements remains uncertain. To address this uncertainty, we compare fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS) measurements of the binding kinetics of a transcription fact...

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Détails bibliographiques
Auteurs principaux: Stasevich, Timothy J., Mueller, Florian, Michelman-Ribeiro, Ariel, Rosales, Tilman, Knutson, Jay R., McNally, James G.
Format: Artigo
Langue:Inglês
Publié: The Biophysical Society 2010
Sujets:
Spectroscopy, Imaging, and Other Techniques
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2966010/
https://ncbi.nlm.nih.gov/pubmed/21044608
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.08.059
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