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Cross-Validating FRAP and FCS to Quantify the Impact of Photobleaching on In Vivo Binding Estimates
Binding can now be quantified in live cells, but the accuracy of such measurements remains uncertain. To address this uncertainty, we compare fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS) measurements of the binding kinetics of a transcription fact...
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| Auteurs principaux: | , , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
The Biophysical Society
2010
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2966010/ https://ncbi.nlm.nih.gov/pubmed/21044608 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.08.059 |
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