Cross-Validating FRAP and FCS to Quantify the Impact of Photobleaching on In Vivo Binding Estimates

Binding can now be quantified in live cells, but the accuracy of such measurements remains uncertain. To address this uncertainty, we compare fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS) measurements of the binding kinetics of a transcription fact...

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Bibliographische Detailangaben
Hauptverfasser: Stasevich, Timothy J., Mueller, Florian, Michelman-Ribeiro, Ariel, Rosales, Tilman, Knutson, Jay R., McNally, James G.
Format: Artigo
Sprache:Inglês
Veröffentlicht: The Biophysical Society 2010
Schlagworte:
Spectroscopy, Imaging, and Other Techniques
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2966010/
https://ncbi.nlm.nih.gov/pubmed/21044608
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.08.059
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