Direct Measurement of Association and Dissociation Rates of DNA Binding in Live Cells by Fluorescence Correlation Spectroscopy

Measurement of live-cell binding interactions is vital for understanding the biochemical reactions that drive cellular processes. Here, we develop, characterize, and apply a new procedure to extract information about binding to an immobile substrate from fluorescence correlation spectroscopy (FCS) a...

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Main Authors: Michelman-Ribeiro, Ariel, Mazza, Davide, Rosales, Tilman, Stasevich, Timothy J., Boukari, Hacene, Rishi, Vikas, Vinson, Charles, Knutson, Jay R., McNally, James G.
Format: Artigo
Sprog:Inglês
Udgivet: The Biophysical Society 2009
Fag:
Spectroscopy, Imaging, and Other Techniques
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2711375/
https://ncbi.nlm.nih.gov/pubmed/19580772
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.04.027
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