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Determination of Dissociation Constants in Living Zebrafish Embryos with Single Wavelength Fluorescence Cross-Correlation Spectroscopy

The quantification of biological interactions is very important in life sciences. Here we report for the first time, to our knowledge, the determination of a biomolecular dissociation constant (K(D)) in living zebrafish embryos at physiological protein expression levels. For that purpose, we extend...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Shi, Xianke, Foo, Yong Hwee, Sudhaharan, Thankiah, Chong, Shang-Wei, Korzh, Vladimir, Ahmed, Sohail, Wohland, Thorsten
Format: Artigo
Sprache:Inglês
Veröffentlicht: The Biophysical Society 2009
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2711317/
https://ncbi.nlm.nih.gov/pubmed/19619483
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.05.006
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