Determination of Dissociation Constants in Living Zebrafish Embryos with Single Wavelength Fluorescence Cross-Correlation Spectroscopy

The quantification of biological interactions is very important in life sciences. Here we report for the first time, to our knowledge, the determination of a biomolecular dissociation constant (K(D)) in living zebrafish embryos at physiological protein expression levels. For that purpose, we extend...

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Hlavní autoři: Shi, Xianke, Foo, Yong Hwee, Sudhaharan, Thankiah, Chong, Shang-Wei, Korzh, Vladimir, Ahmed, Sohail, Wohland, Thorsten
Médium: Artigo
Jazyk:Inglês
Vydáno: The Biophysical Society 2009
Témata:
Spectroscopy, Imaging, and Other Techniques
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2711317/
https://ncbi.nlm.nih.gov/pubmed/19619483
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.05.006
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