Determination of in Vivo Dissociation Constant, K(D), of Cdc42-Effector Complexes in Live Mammalian Cells Using Single Wavelength Fluorescence Cross-correlation Spectroscopy

The RhoGTPase Cdc42 coordinates cell morphogenesis, cell cycle, and cell polarity decisions downstream of membrane-bound receptors through distinct effector pathways. Cdc42-effector protein interactions represent important elements of cell signaling pathways that regulate cell biology in systems as...

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Detalhes bibliográficos
Main Authors: Sudhaharan, Thankiah, Liu, Ping, Foo, Yong Hwee, Bu, Wenyu, Lim, Kim Buay, Wohland, Thorsten, Ahmed, Sohail
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2009
Assuntos:
Mechanisms of Signal Transduction
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2679461/
https://ncbi.nlm.nih.gov/pubmed/19293156
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M900894200
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