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Synthesis of 5′ cap-0 and cap-1 RNAs using solid-phase chemistry coupled with enzymatic methylation by human (guanine-N(7))-methyl transferase

The 5′ end of eukaryotic mRNA carries a N(7)-methylguanosine residue linked by a 5′-5′ triphosphate bond. This cap moiety ((7m)GpppN) is an essential RNA structural modification allowing its efficient translation, limiting its degradation by cellular 5′ exonucleases and avoiding its recognition as “...

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書誌詳細
主要な著者: Thillier, Yann, Decroly, Etienne, Morvan, François, Canard, Bruno, Vasseur, Jean-Jacques, Debart, Françoise
フォーマット: Artigo
言語:Inglês
出版事項: Cold Spring Harbor Laboratory Press 2012
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3312571/
https://ncbi.nlm.nih.gov/pubmed/22334760
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.030932.111
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