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Evidence for an intermediate conformational state of LacY

LacY mutant Cys154 → Gly exhibits a periplasmic-closed crystal structure identical to the WT, but is periplasmic-open in the membrane. The mutant hardly catalyzes transport, but binds galactosides from either side of the membrane with the same affinity and is resistant to site-directed proteolysis r...

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Detalhes bibliográficos
Main Authors: Jiang, Xiaoxu, Guan, Lan, Zhou, Yonggang, Hong, Wen-Xu, Zhang, Qinghai, Kaback, H. Ronald
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2012
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3311394/
https://ncbi.nlm.nih.gov/pubmed/22355148
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1201107109
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