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Purification, crystallization and preliminary X-ray diffraction analysis of the Fyn SH2 domain and its complex with a phosphotyrosine peptide

SH2 domains are widespread protein-binding modules that recognize phosphotyrosines and play central roles in intracellular signalling pathways. The SH2 domain of the human protein tyrosine kinase Fyn has been expressed, purified and crystallized in the unbound state and in complex with a high-affini...

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Detalhes bibliográficos
Main Authors: Huculeci, Radu, Buts, Lieven, Lenaerts, Tom, van Nuland, Nico A. J., Garcia-Pino, Abel
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3310552/
https://ncbi.nlm.nih.gov/pubmed/22442244
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112004186
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