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End-Point Immobilization of Recombinant Thrombomodulin via Sortase-Mediated Ligation
We report an enzymatic end-point modification and immobilization of recombinant human thrombomodulin (TM), a cofactor for activation of anticoagulant protein C pathway via thrombin. First, a truncated TM mutant consisting of epidermal growth factor-like domains 4–6 (TM(456)) with a conserved pentape...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3310247/ https://ncbi.nlm.nih.gov/pubmed/22372933 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bc200661w |
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