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Restriction enzyme-free construction of random gene mutagenesis libraries in E. coli
Directed evolution relies on both random and site-directed mutagenesis of individual genes and regulatory elements to create variants with altered activity profiles for engineering applications. Central to these experiments is the construction of large libraries of related variants. However, a numbe...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2011
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3274633/ https://ncbi.nlm.nih.gov/pubmed/22155067 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.11.009 |
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