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Designing randomized DNA sequences free of restriction enzyme recognition sites

DNA libraries containing random “barcodes” complicate synthetic biology workflows that utilize restriction enzymes, since restriction sites can appear inside some barcodes. By removing bases at particular sites in the barcodes, it is possible to create semi-random pools of barcodes that do not conta...

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Bibliografiska uppgifter
I publikationen:Biotechnol J
Huvudupphovsmän: Storm, Audra J., Jensen, Paul A.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2017
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC5762418/
https://ncbi.nlm.nih.gov/pubmed/28865135
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/biot.201700326
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