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Restriction enzyme-free mutagenesis via the light regulation of DNA polymerization
The effects of photocaged nucleosides on the DNA polymerization reaction was investigated, finding that most polymerases are unable to recognize and read through the presence of a single caging group on the DNA template. Based on this discovery, a new method of introducing mutations into plasmid DNA...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2677887/ https://ncbi.nlm.nih.gov/pubmed/19293272 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp150 |
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