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Restriction enzyme-free construction of random gene mutagenesis libraries in E. coli

Directed evolution relies on both random and site-directed mutagenesis of individual genes and regulatory elements to create variants with altered activity profiles for engineering applications. Central to these experiments is the construction of large libraries of related variants. However, a numbe...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Pai, Jen C., Entzminger, Kevin C., Maynard, Jennifer A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2011
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3274633/
https://ncbi.nlm.nih.gov/pubmed/22155067
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.11.009
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