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Construction and Analysis of a Library for Random Insertional Mutagenesis in Streptococcus pneumoniae: Use for Recovery of Mutants Defective in Genetic Transformation and for Identification of Essential Genes

To explore the use of insertion-duplication mutagenesis (IDM) as a random gene disruption mutagenesis tool for genomic analysis of Streptococcus pneumoniae, a large mutagenic library of chimeric plasmids with 300-bp inserts was constructed. The library was large enough to produce 60,000 independent...

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Detalhes bibliográficos
Main Authors: Lee, Myeong S., Dougherty, Brian A., Madeo, Anne C., Morrison, Donald A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 1999
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC91271/
https://ncbi.nlm.nih.gov/pubmed/10223974
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