Construction and Analysis of a Library for Random Insertional Mutagenesis in Streptococcus pneumoniae: Use for Recovery of Mutants Defective in Genetic Transformation and for Identification of Essential Genes

To explore the use of insertion-duplication mutagenesis (IDM) as a random gene disruption mutagenesis tool for genomic analysis of Streptococcus pneumoniae, a large mutagenic library of chimeric plasmids with 300-bp inserts was constructed. The library was large enough to produce 60,000 independent...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Lee, Myeong S., Dougherty, Brian A., Madeo, Anne C., Morrison, Donald A.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1999
Assuntos:	Genetics and Molecular Biology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91271/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10223974/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.5.1883-1890.1999
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