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Isolation of transformation-deficient Streptococcus pneumoniae mutants defective in control of competence, using insertion-duplication mutagenesis with the erythromycin resistance determinant of pAM beta 1.

Several transformation-deficient mutants of Streptococcus pneumoniae were isolated after insertion-duplication mutagenesis. Mutagenesis was accomplished by transformation of competent cells with chimeric DNA formed by the ligation of TaqI fragments of pneumococcal DNA to the erythromycin resistance...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Morrison, D A, Trombe, M C, Hayden, M K, Waszak, G A, Chen, J D
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1984
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215739/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6090396/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.159.3.870-876.1984
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