Isolation of transformation-deficient Streptococcus pneumoniae mutants defective in control of competence, using insertion-duplication mutagenesis with the erythromycin resistance determinant of pAM beta 1.

Several transformation-deficient mutants of Streptococcus pneumoniae were isolated after insertion-duplication mutagenesis. Mutagenesis was accomplished by transformation of competent cells with chimeric DNA formed by the ligation of TaqI fragments of pneumococcal DNA to the erythromycin resistance...

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Main Authors: Morrison, D A, Trombe, M C, Hayden, M K, Waszak, G A, Chen, J D
Formato: Artigo
Idioma:Inglês
Publicado: 1984
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC215739/
https://ncbi.nlm.nih.gov/pubmed/6090396
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