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Monitoring protein stability and aggregation in vivo by real-time fluorescent labeling
In vivo fluorescent labeling of an expressed protein has enabled the observation of its stability and aggregation directly in bacterial cells. Mammalian cellular retinoic acid-binding protein I (CRABP I) was mutated to incorporate in a surface-exposed omega loop the sequence Cys-Cys-Gly-Pro-Cys-Cys,...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
2004
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC327180/ https://ncbi.nlm.nih.gov/pubmed/14701904 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0304533101 |
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