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Monitoring protein stability and aggregation in vivo by real-time fluorescent labeling

In vivo fluorescent labeling of an expressed protein has enabled the observation of its stability and aggregation directly in bacterial cells. Mammalian cellular retinoic acid-binding protein I (CRABP I) was mutated to incorporate in a surface-exposed omega loop the sequence Cys-Cys-Gly-Pro-Cys-Cys,...

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Bibliografske podrobnosti
izdano v:Proc Natl Acad Sci U S A
Main Authors: Ignatova, Zoya, Gierasch, Lila M.
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2003
Teme:
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327180/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14701904/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.0304533101
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