Deep Amino Acid Sequencing of Native Brain GABA(A) Receptors Using High-Resolution Mass Spectrometry

Mass spectrometric sequencing of low abundance, integral membrane proteins, particularly the transmembrane domains, presents challenges that span the multiple phases of sample preparation including solubilization, purification, enzymatic digestion, peptide extraction, and chromatographic separation....

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Chen, Zi-Wei, Fuchs, Karoline, Sieghart, Werner, Townsend, R. Reid, Evers, Alex S.
Format: Artigo
Sprache:Inglês
Veröffentlicht: The American Society for Biochemistry and Molecular Biology 2012
Schlagworte:
Research
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3270104/
https://ncbi.nlm.nih.gov/pubmed/22338125
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M111.011445
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