Biotin and fluorescent labeling of RNA using T4 RNA ligase.
Biotin, fluorescein, and tetramethylrhodamine derivatives of P1-(6-aminohex-1-yl)-P2-(5'-adenosine) pyrophosphate were synthesized and used as substrates with T4 RNA ligase. In the absence of ATP, the non-adenylyl portion of these substrates is transferred to the 3'-hydroxyl of an RNA acceptor to fo...
Guardat en:
| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1983
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326365/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6194506/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.18.6167 |
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