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Biotin and fluorescent labeling of RNA using T4 RNA ligase.

Biotin, fluorescein, and tetramethylrhodamine derivatives of P1-(6-aminohex-1-yl)-P2-(5'-adenosine) pyrophosphate were synthesized and used as substrates with T4 RNA ligase. In the absence of ATP, the non-adenylyl portion of these substrates is transferred to the 3'-hydroxyl of an RNA acce...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Richardson, R W, Gumport, R I
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 1983
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326365/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6194506/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.18.6167
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