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Synthesis and reactivity of intermediates formed in the T4 RNA ligase reaction.
The intermediate adenylated donor derivatives A(5')pp(5')dTp and A(5')pp(5')GpGpGp have been prepared from suitable phosphorylating reagents activated by 1-hydroxybenzotriazole. Phosphodiester bond formation between donor and acceptor oligonucleotides as catalyzed by T4 RNA ligas...
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| Autores principales: | , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
1987
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| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC305962/ https://ncbi.nlm.nih.gov/pubmed/3299268 |
| Etiquetas: |
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