Synthesis and reactivity of intermediates formed in the T4 RNA ligase reaction.
The intermediate adenylated donor derivatives A(5')pp(5')dTp and A(5')pp(5')GpGpGp have been prepared from suitable phosphorylating reagents activated by 1-hydroxybenzotriazole. Phosphodiester bond formation between donor and acceptor oligonucleotides as catalyzed by T4 RNA ligase is shown to be mor...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1987
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| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC305962/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3299268/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.13.5289 |
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