Synthesis and reactivity of intermediates formed in the T4 RNA ligase reaction.

The intermediate adenylated donor derivatives A(5')pp(5')dTp and A(5')pp(5')GpGpGp have been prepared from suitable phosphorylating reagents activated by 1-hydroxybenzotriazole. Phosphodiester bond formation between donor and acceptor oligonucleotides as catalyzed by T4 RNA ligase is shown to be mor...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Hoffmann, P U, McLaughlin, L W
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1987
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC305962/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3299268/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.13.5289
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