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Reactions at the termini of tRNA with T4 RNA ligase.

T4 RNA ligase will catalyze the addition of nucleoside 3', 5'-bisphosphates onto the 3' terminus of tRNA resulting in tRNA molecule one nucleotide longer with a 3' terminal phosphate. Under appropriate conditions the reaction is quantitative and, if high specific radioactivity bi...

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Autors principals: Bruce, A G, Uhlenbeck, O C
Format: Artigo
Idioma:Inglês
Publicat: 1978
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC342702/
https://ncbi.nlm.nih.gov/pubmed/724498
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