Reactions at the termini of tRNA with T4 RNA ligase.
T4 RNA ligase will catalyze the addition of nucleoside 3', 5'-bisphosphates onto the 3' terminus of tRNA resulting in tRNA molecule one nucleotide longer with a 3' terminal phosphate. Under appropriate conditions the reaction is quantitative and, if high specific radioactivity bisphosphates are used...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1978
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| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342702/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/724498/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.10.3665 |
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