Loading...

Biotin and fluorescent labeling of RNA using T4 RNA ligase.

Biotin, fluorescein, and tetramethylrhodamine derivatives of P1-(6-aminohex-1-yl)-P2-(5'-adenosine) pyrophosphate were synthesized and used as substrates with T4 RNA ligase. In the absence of ATP, the non-adenylyl portion of these substrates is transferred to the 3'-hydroxyl of an RNA acce...

Full description

Saved in:
Bibliographic Details
Main Authors: Richardson, R W, Gumport, R I
Format: Artigo
Language:Inglês
Published: 1983
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC326365/
https://ncbi.nlm.nih.gov/pubmed/6194506
Tags: Add Tag
No Tags, Be the first to tag this record!