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Energetics of Cyclic AMP Binding to HCN Channel C Terminus Reveal Negative Cooperativity

Cyclic AMP binds to the HCN channel C terminus and variably stabilizes its open state. Using isothermal titration calorimetry, we show that cAMP binds to one subunit of tetrameric HCN2 and HCN4 C termini with high affinity (∼0.12 μm) and subsequently with low affinity (∼1 μm) to the remaining three...

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Hlavní autoři: Chow, Sarah S., Van Petegem, Filip, Accili, Eric A.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Biochemistry and Molecular Biology 2012
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3249114/
https://ncbi.nlm.nih.gov/pubmed/22084239
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.269563
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