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Identification and remediation of biases in the activity of RNA ligases in small-RNA deep sequencing

Deep sequencing of small RNAs (sRNA-seq) is now the gold standard for small RNA profiling and discovery. Biases in sRNA-seq have been reported, but their etiology remains unidentified. Through a comprehensive series of sRNA-seq experiments, we establish that the predominant cause of the bias is the...

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Hlavní autoři: Jayaprakash, Anitha D., Jabado, Omar, Brown, Brian D., Sachidanandam, Ravi
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2011
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3241666/
https://ncbi.nlm.nih.gov/pubmed/21890899
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr693
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