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Structural bias in T4 RNA ligase-mediated 3′-adapter ligation
T4 RNA ligases are commonly used to attach adapters to RNAs, but large differences in ligation efficiency make detection and quantitation problematic. We developed a ligation selection strategy using random RNAs in combination with high-throughput sequencing to gain insight into the differences in e...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3326334/ https://ncbi.nlm.nih.gov/pubmed/22241775 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr1263 |
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