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Bias in Ligation-Based Small RNA Sequencing Library Construction Is Determined by Adaptor and RNA Structure

High-throughput sequencing (HTS) has become a powerful tool for the detection of and sequence characterization of microRNAs (miRNA) and other small RNAs (sRNA). Unfortunately, the use of HTS data to determine the relative quantity of different miRNAs in a sample has been shown to be inconsistent wit...

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Bibliografische gegevens
Gepubliceerd in:PLoS One
Hoofdauteurs: Fuchs, Ryan T., Sun, Zhiyi, Zhuang, Fanglei, Robb, G. Brett
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Public Library of Science 2015
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4420488/
https://ncbi.nlm.nih.gov/pubmed/25942392
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0126049
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