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Bias in Ligation-Based Small RNA Sequencing Library Construction Is Determined by Adaptor and RNA Structure

High-throughput sequencing (HTS) has become a powerful tool for the detection of and sequence characterization of microRNAs (miRNA) and other small RNAs (sRNA). Unfortunately, the use of HTS data to determine the relative quantity of different miRNAs in a sample has been shown to be inconsistent wit...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Fuchs, Ryan T., Sun, Zhiyi, Zhuang, Fanglei, Robb, G. Brett
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4420488/
https://ncbi.nlm.nih.gov/pubmed/25942392
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0126049
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