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Sequencing bias: comparison of different protocols of MicroRNA library construction
BACKGROUND: MicroRNAs(miRNAs) are 18-25 nt small RNAs playing critical roles in many biological processes. The majority of known miRNAs were discovered by conventional cloning and a Sanger sequencing approach. The next-generation sequencing (NGS) technologies enable in-depth characterization of the...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
BioMed Central
2010
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2946280/ https://ncbi.nlm.nih.gov/pubmed/20815927 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-10-64 |
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