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Splint ligation of RNA with T4 DNA ligase
Splint ligation of RNA, whereby specific RNA molecules are ligated together, can be carried out using T4 DNA ligase and a bridging DNA oligonucleotide complementary to the RNAs. This method takes advantage of the property of T4 DNA ligase to join RNA molecules when they are in an RNA:DNA hybrid. Spl...
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| Publicado no: | Methods Mol Biol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4234903/ https://ncbi.nlm.nih.gov/pubmed/23065567 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-113-4_19 |
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