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Splint ligation of RNA with T4 DNA ligase

Splint ligation of RNA, whereby specific RNA molecules are ligated together, can be carried out using T4 DNA ligase and a bridging DNA oligonucleotide complementary to the RNAs. This method takes advantage of the property of T4 DNA ligase to join RNA molecules when they are in an RNA:DNA hybrid. Spl...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Methods Mol Biol
Main Authors: Kershaw, Christopher J., O’Keefe, Raymond T.
Format: Artigo
Jezik:Inglês
Izdano: 2012
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4234903/
https://ncbi.nlm.nih.gov/pubmed/23065567
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-113-4_19
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