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Efficient DNA ligation in DNA–RNA hybrid helices by Chlorella virus DNA ligase
Single-stranded DNA molecules (ssDNA) annealed to an RNA splint are notoriously poor substrates for DNA ligases. Herein we report the unexpectedly efficient ligation of RNA-splinted DNA by Chlorella virus DNA ligase (PBCV-1 DNA ligase). PBCV-1 DNA ligase ligated ssDNA splinted by RNA with k(cat) ≈ 8...
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| Autori principali: | , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
2014
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3919565/ https://ncbi.nlm.nih.gov/pubmed/24203707 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt1032 |
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